Rapid Simultaneous Quantitative Analysis of Polygalaxanthone III and 3,6'-Disinapoyl Sucrose in Polygala Radix

Abstract

Polygala Radix ("Onji") is an important crude drug used in Kampo formulation. So far, there is only one established quantitative analytical method for separating the impurities of polygalaxanthone III from Polygara Radix. However, it is difficult to simultaneously perform quantitative analysis for 3,6'-disinapoyl sucrose with polygalaxanthone III, because the analysis time is very long (retention time; 240 min). Therefore, our aim is to develop an ultra high-performance liquid chromatography technique that can be used with a very short analytical time (retention time; 27 min). We hope that this method will become an important and effective means for conducting on-site quality assessments in the future.