BUNSEKI KAGAKU
Print ISSN : 0525-1931
Research Papers
Solid-phase Extraction Properties of Novel Sorbents Modified with Adenine for Nucleic Acid Related Compounds
Yuta MIKIHiroya MURAKAMIMiho OMIYAYukihiro ESAKAYoshinori INOUENorio TESHIMA
Author information
JOURNALS FREE ACCESS

2018 Volume 67 Issue 8 Pages 445-451

Details
Abstract

Adenine-modified hydrophilic sorbents were synthesized and evaluated by solid-phase extraction (SPE) with several modes, namely HILIC, reversed phase and nonaqueous. To obtain such novel sorbents, adenine was modified into a hydrophilic base resin that was a copolymer of glycidyl methacrylate (GMA) with ethylene glycol dimethacrylate (EGDM) or glycerol dimethacrylate (GDMA). The specific sorptive properties for cytidine and guanosine under the HILIC SPE mode were observed in both Adenine-modified sorbents of GMA and EGDM (Adenine-EGDM) and of GMA and GDMA, while such sorptive properties were not observed in each unmodified sorbent. On the other hand, the specific sorptive properties under the reversed phase SPE mode were not obtained in both Adenine-modified sorbents. These results indicated that the Adenine-modified sorbents probably have the property of hydrogen bonding derived from adenine. To study the influence of hydrogen bonding derived from adenine, the sorptive properties were investigated under the nonaqueous SPE mode. Improvements of the sorptive properties for uridine, cytidine and guanosine were observed in Adenine-EGDM in this mode, which indicated that an enhancement of the property of hydrogen bonding could be occur. These Adenine-modified sorbents have the selectivity for nucleic acids and nucleosides.

Information related to the author
© 2018 The Japan Society for Analytical Chemistry
Next article
feedback
Top