BUNSEKI KAGAKU
Print ISSN : 0525-1931
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Development of an LC-NMR System Using a Diode Array Detector and a Charged Aerosol Detector
Yayoi KANBAYASHIYu TSUTSUMIToshimi MIZUKOSHIHideyuki YAMAGUCHI
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2020 Volume 69 Issue 3 Pages 151-155

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Abstract

Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for structure determination. However, NMR spectra sometimes suffer from severe signal overlaps. To avoid such signal overlaps, LC-DAD-NMR systems have been used. These systems have enabled the separation of components on a column before NMR detection. However, DAD detection alone is insufficient when the sample does not contain chromophores. To increase the detection coverage, we used a corona charged aerosol detector (CAD) that responded to analytes independent of their physical/chemical characteristics. The established LC-DAD/CAD NMR system was evaluated. Its calculated detection and quantitation limits were 0.25 and 1.0 μg/peak, respectively. This system was used to analyze rubus extract. Along with glucose and fructose, a small amount of rubusoside was detected by corona CAD and identified by NMR without needing a chromophore.

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© 2020 The Japan Society for Analytical Chemistry
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