Butsuri
Online ISSN : 2423-8872
Print ISSN : 0029-0181
ISSN-L : 0029-0181
Interdisciplinary
Two-Dimensional Spectroscopy: With a Focus on Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy for Visualizing Protein Dynamics
Kunihiko IshiiTahei Tahara
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2017 Volume 72 Issue 12 Pages 854-861

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Abstract

Two-dimensional spectroscopy is an emerging topic in the field of spectroscopy. By mapping correlations of two spectroscopic measurements, two-dimensional spectroscopy resolves complexity buried in the signal from complex and/or fluctuating molecular systems. We first outline the basic concepts of two-dimensional spectroscopy and then discuss a new single-molecule based method, two-dimensional fluorescence lifetime correlation spectroscopy, which visualizes microsecond protein dynamics.

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© 2017 The Physical Society of Japan
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