2017 Volume 29 Issue 1 Pages 80-83
Even if the newt removes lens surgically, the lens is regeneration at the dedifferentiation of iris pigment epithelium cell. Authors introduced the episomal vector into human iris tissue stem / progenitor cells and created induced pluripotent stem cells (iPS cells). The iPS cells derived from the iris were with positive at Alkaline phosphatase (ALP), Sox2 and SSEA-4. The iPS cells differentiated to lens epithelial cells using the rotary floating culture method. This result increased the αA-crystalline gene which was one of the markers of lens epithelial cells than the derivative previous method using the embryonic stem cells, and the cell aggregation which expressed the αA-crystalline protein was formed.