The Journal of The Japanese Society for Cataract Research
Online ISSN : 2188-613X
Print ISSN : 0915-4302
ISSN-L : 0915-4302
Articles
Differentiation to the Lens Epithelial Cells Using Induced Pluripotent Stem Cells Derived from Human Iris
Naoki YamamotoNoriko HiramatsuAtsuhiro TanikawaEi-ichi MiyachiMasayuki Horiguchi
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2017 Volume 29 Issue 1 Pages 80-83

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Abstract

Even if the newt removes lens surgically, the lens is regeneration at the dedifferentiation of iris pigment epithelium cell. Authors introduced the episomal vector into human iris tissue stem / progenitor cells and created induced pluripotent stem cells (iPS cells). The iPS cells derived from the iris were with positive at Alkaline phosphatase (ALP), Sox2 and SSEA-4. The iPS cells differentiated to lens epithelial cells using the rotary floating culture method. This result increased the αA-crystalline gene which was one of the markers of lens epithelial cells than the derivative previous method using the embryonic stem cells, and the cell aggregation which expressed the αA-crystalline protein was formed.

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© 2017 Japanese Society for Cataract Research
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