2018 Volume 30 Issue 1 Pages 13-21
Lutein is indicated eﬀective on preventing age-related macular degeneration while epidemiological surveys on inhibiting cataracts did not reach the consensus. Confounding factors, such as individual diﬀerences and the changes in amount of daily intake, may interfere with the results. We investigated the changes in oxidative status after administering supplement which containing determined dose of antioxidants by measuring samples from patients with identical grades of cataracts in both eyes. By this method, the individual diﬀerences and the changes in daily intake can be controlled. Aqueous humor and the lenticular anterior capsule samples were collected during cataract surgery before six-week oral administration of Ocuvite+Lutein®, an antioxidant supplement, as pre-intake. Post-intake samples were collected with the same method during cataract surgery of the opposite eye after the supplementation. The superoxide scavenging activity increased while total hydroperoxides decreased in aqueous humor after the supplementation. In the lenticular anterior capsule, the expression of glucose-6-phosphate dehydorgenase, a rate-determining enzyme of the pentose phosphate cycle, and 18S rRNA, related to protein synthesis in ribosome, increased. On the other hand, the expression of Aquaporin 8, a diﬀusion facilitator of hydrogen peroxide, decreased. According to the results of this study, the lutein-containing supplement suppresses the oxidation in the lens, and the potential in preventing the onset and the progression of cataracts was suggested.