The Journal of The Japanese Society for Cataract Research
Online ISSN : 2188-613X
Print ISSN : 0915-4302
ISSN-L : 0915-4302
JSCR Award Lecture 2019
The Role of Tropomyosin in Lens Epithelial Mesenchymal Transition and Differentiation
Teppei Shibata
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2020 Volume 32 Issue 1 Pages 7-10

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Abstract

One of the most common complications after cataract surgery is a posterior capsule opacity (also called posterior capsule opacification or PCO). We have focused that Tropomyosin (Tpm) may be involved in PCO development. In this study, we generated Tpm1 conditional knock-out mice (Tpm1-CKO) and Tpm2 hetero knock-out mice (Tpm2+/-) and observed their age-related histological changes to investigate their role in lens development and differentiation. In addition, differences in epithelial mesenchymal transition (EMT) induction were observed in wound healing models using Tpm2+/-. In Tpm2+/-, lens vacuoles were observed at 14 weeks of age. Lens, swelling and liquefaction of lens fibers were observed at Tpm2+/-compared to WT at 53 and 83 weeks of age. In the wound healing models, EMT change was suppressed in Tpm2+/-compared to WT. At Tpm1-/-, the lens was small and abnormal fiber differentiation was observed from 1 week of age, and liquefaction and swelling of lens fibers were progressed with aging. Tpm1 and 2 may not only be involved in EMT of PCO, but may also be involved in the normal lens development and differentiation.

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© 2020 Japanese Society for Cataract Research
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