2010 年 30 巻 3 号 p. 165-170
We studied the interaction of a β-adrenergic receptor agonist and an adenylyl cyclase activator with volatile anesthetics in combination with nitrous oxide (N2O). The whole-cell patch clamp technique was used to measure the amplitude of L-type Ca2＋ channel current(ICa,L) in rabbit ventricular myocytes. The effects of sevoflurane or halothane in combination with N2O were compared with their effects in the presence of β-adrenoceptor stimulation by isoproterenol or adenylyl cyclase activation by forskolin. The effects of sevoflurane plus N2O and halothane plus N2O under basal conditions significantly differed from their effects in the presence of isoproterenol. Isoproterenol and forskolin diminished the ability of two gas combinations to suppress ICa,L. Our findings suggest that both sevoflurane and halothane in the presence of N2O modulate the catecholamine sensitivity of the β-adrenergic cascade.