LETHALITY AND TERATOGENICITY OF CYCLOPHOSPHAMIDE (ENDOXAN-ASTA) IN CHICK EMBRYOS
1972 Volume 12 Issue 2 Pages 61-72
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1972 Volume 12 Issue 2 Pages 61-72
1. Cyclophosphamide (endoxan-asta) was injected into the yolk sac of chick embryos on the 3rd through the 6th days of incubation in doses varying from 0.035 mg to 0.08 mg and was found to be more lethal than teratogenic. 2. Mortality rate of the chicks was greater when the drug was given earlier in development (51.2% in the 3rd day injections and 16% in the 6th day group). Higher doses (0.08 mg on 5th day) caused 86. 6% mortality. 3. Teratogenic effects produced a spectrum of malformations resulting in beak defects, eye defects. exencephaly/encephalocele, deformities of limbs and toes, rudimentary wings, ectopia viscerum, skeletal defects and stunting of growth. These show marked resemblance to the malformations produced by cyclophosphamide in rats and mice. 4. Dose of 0.05 mg on 5th day deformed maximum number of chicks (27. 45).
