official journal of Congeital Anomalies Research Association of Japan
Online ISSN : 2433-1503
Print ISSN : 0037-2285
IN-VITRO CHROMOSOMAL RADIOSENSITIVITY IN PATIENTS WITH CHROMOSOMAL ABNORMALITIES
Makoto HIGURASHIPatrick E. CONEN
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1972 Volume 12 Issue 2 Pages 113-119

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Abstract

This paper reports results of the in vitro tests of chromosomal radiosensitivity in ten children and three adults with normal karyotypes as controls, 13 patients (children) with chromosomal abnormality and six translocation carriers (adults). Peripheral blood samples from each individual and skin fibroblast cultures from eight control children, nine patients with chromosomal abnormalities and one translocation carriers were irradiated with 10 and 100 rads. Non-irradiated duplicate cultures were used as contrlos. Blood cultures were harvested after 52 hours of incubation, while fibroblast cultures were harvested 24 hours after irradiation. The frequency of chromosomal breakage caused by irradiation was calculated by subtracting figures for non-irradiated samples from those for irradiated samples. The number of chromosome-type breaks per cell/rad in normal controls was 0.0023±0.0010 for children and 0.0020±0.0009 for adults in lymphocytes, and 0.0048±0.0016 for fibroblasts from children. In patients the number of breaks per cell/rad was 0.0051±0.0015 in lymphocytes and 0.0081±0.0017 in fibroblasts, and in carriers was 0.0040±0.0018 in lymphocytes and 0.0063±0.0017 in fibroblasts. In comparison with age-matched controls, significantly elevated frequencies of breaks per cell/rad were observed in cultured lymphocytes and fibroblasts of patients, and in cultured lymphocytes of translocation carriers. After irradiation with 100 rads the number of dicentrics and rings per cell was significantly greater in both patients and carriers of chromosomal abnormalities than in controls. Threrfore, chromosomes of cells from patients with chromosomal abnormalities and translocation carriers were significantly more radiosensitive in vitro than those of controls.

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© 1972 The Japanese Teratology Society
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