Abstract
Effects of cefamandole sodium and cefazolin sodium administrations on several enzyme activities in plasma and liver were examined in rats. Marked decrease in plasma glutamic pyruvic transaminase activity was observed 15-30 min. after the intravenous administration of cefamandale and the activity recovered to normal range 2 hrs. later. On the other hand, the activity of glutamic pyruvic transaminase in plasma depressed gradually following the administration of cefazolin, and lower activity was observed even at 1-2days later. Successive administrations of cefazolin for 2-3 days resulted in decrease of the glutamic pyruvic transaminase activity in both plasma and liver, but not by the administration of cefamandole. However, other enzyme activities in plasma and liver were not affected by the administration of cefamandole and cefazolin.
Glutamic pyruvic transaminase activity in liver cytosol was inhibited by adding cefamandole and cefazolin to the reaction mixture, suggesting direct interaction of cefamandole and cefazolin with the enzyme followed by inhibition of the enzyme activity.
