Abstract
We studied the effect of combined therapy with anti-cancer and immunotherapeutic agents on transplantable tumor in rats. After treatment with polysaccharide-K (PSK)(300/mg/day for 4 days) and cyclophosphamide (CPA)(20mg/mg/day for 1 day), macrophages from the bone marrow and spleen of Donryu rats were incubated in a liquid culture medium. The number of colonies and macrophage clusters was then counted. The number was greatest in the group given PSK followed by CPA. Treatment with CPA followed by PSK caused no increase in number.
Survivals were observed in the groups inoculated with Yoshida sarcoma cells in the intra-abdominal cavity and given CPA and/or PSK.
In the groups given CPA before the transplantation of tumor cells, survival time was shorter than in the untreated group.
Treatment with CPA followed by PSK induced the longest survival time as compared to single treatment with CPA or combined treatment with PSK followed by CPA.
