マウス乳腺発育におけるProlactinおよびGrowth hormoneの重要性

矢内 玲子; 長澤 弘

doi:10.2508/chikusan.42.31

矢内玲子, 長澤弘

著者情報

ジャーナルフリー

42 巻 (1971) 1 号 p. 31-38

DOI https://doi.org/10.2508/chikusan.42.31

詳細

公開日
公開日: 2008/03/10 [早期公開] 公開日: - 受付日: 1970/08/26 改訂日: - 受理日: -
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訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : The effects of prolactin (PL) and/or growth hormone (GH) on mammary gland development in adrenalectomized-ovariectomized mice were investigated to examine how these mammotropins influence the gland in the apparent absence of steroid hormones.
Animals used were C3H/He strain of female mice which had been bred in the authors' laboratory. They were divided into 5 groups consisted of 8 each at about 70 and 55 days of age in Experiments 1 and 2, respectively. In both experiments, group I was given no treatment and served as the intact control, and groups II-V consisted of adrenalectomized-ovariectomized animals. Group II was given no injection and served as the adrenalectomized-ovariec-
tomized control. Groups III, IV and V received subcutaneous injections of 0.5 mg of PL (NIHP-B₂), 1 mg of GH (NIH-GH-B₁₂) and PL+GH twice daily, respectively. In Experiment 1, the injection was given for 7 days from the following morning of the operation of adrenalectomy and ovariectomy. In Experiment 2, it was given for 10 days beginning 7 days after the operation in order to examine the effects of these hormones on the once involuted mammary glands. All mice were killed on the morning following the last injections and the left third thoracic and the bilateral inguinal glands were used for the whole mount preparation and the determination of deoxyribonucleic acid (DNA) content, respectively. The mammary development in
the whole mount preparation was graded from 1 to 4 in increments of 1 for either ductal branching or presence of end-buds.
The increases in body weights were more marked in groups IV and V than in the other 3 groups, especially in Experiment 2 (Table 1).
In Experiment 1, the intact control and all injected groups were significantly superior to group II in the ductal growth, except group IV which had a large variation (Figs. 1 and 2). No significant differences in the degree of the formation of end-buds were observed among groups, but group V appeared to be better than the other 4 groups in this measure. Mammary DNA content was significantly less in group II than in the other 4 groups (Fig. 3). DNA
content in group V was higher than those in groups I and III.
In Experiment 2, ductal developments in groups III, IV and V were more conspicuous than that in group II. Group V was significantly higher than groups I and II in the degree of end-bud formation (Figs. 4 and 5). The well developed end-buds and small alveoli were often observed in group III. The difference in mammary DNA content was not statistically significant among groups, but DNA contents of groups IV and V were comparable to that of group I
(Fig. 6).
In general, mammary gland was considerably involuted by removal of both adrenal glands and ovaries. PL, GH or PL+GH could prevent the gland from involution, and maintain the normal level. Furthermore, GH or PL+GH could restore the gland to the normal level even after the gland was once involuted almost completely. Prolactin was apparently effective on the formation of end-buds. The present results provide the definite evidence that PL and GH are
the primary important mammotropins in mice.
Right : The effects of prolactin (PL) and/or growth hormone (GH) on mammary gland development in adrenalectomized-ovariectomized mice were investigated to examine how these mammotropins influence the gland in the apparent absence of steroid hormones.
Animals used were C3H/He strain of female mice which had been bred in the authors' laboratory. They were divided into 5 groups consisted of 8 each at about 70 and 55 days of age in Experiments 1 and 2, respectively. In both experiments, group I was given no treatment and served as the intact control, and groups II-V consisted of adrenalectomized-ovariectomized animals. Group II was given no injection and served as the adrenalectomized-ovariec-tomized control. Groups III, IV and V received subcutaneous injections of 0.5 mg of PL (NIHP-B₂), 1 mg of GH (NIH-GH-B₁₂) and PL+GH twice daily, respectively. In Experiment 1, the injection was given for 7 days from the following morning of the operation of adrenalectomy and ovariectomy. In Experiment 2, it was given for 10 days beginning 7 days after the operation in order to examine the effects of these hormones on the once involuted mammary glands. All mice were killed on the morning following the last injections and the left third thoracic and the bilateral inguinal glands were used for the whole mount preparation and the determination of deoxyribonucleic acid (DNA) content, respectively. The mammary development in the whole mount preparation was graded from 1 to 4 in increments of 1 for either ductal branching or presence of end-buds.
The increases in body weights were more marked in groups IV and V than in the other 3 groups, especially in Experiment 2 (Table 1).
In Experiment 1, the intact control and all injected groups were significantly superior to group II in the ductal growth, except group IV which had a large variation (Figs. 1 and 2). No significant differences in the degree of the formation of end-buds were observed among groups, but group V appeared to be better than the other 4 groups in this measure. Mammary DNA content was significantly less in group II than in the other 4 groups (Fig. 3). DNA content in group V was higher than those in groups I and III.
In Experiment 2, ductal developments in groups III, IV and V were more conspicuous than that in group II. Group V was significantly higher than groups I and II in the degree of end-bud formation (Figs. 4 and 5). The well developed end-buds and small alveoli were often observed in group III. The difference in mammary DNA content was not statistically significant among groups, but DNA contents of groups IV and V were comparable to that of group I (Fig. 6).
In general, mammary gland was considerably involuted by removal of both adrenal glands and ovaries. PL, GH or PL+GH could prevent the gland from involution, and maintain the normal level. Furthermore, GH or PL+GH could restore the gland to the normal level even after the gland was once involuted almost completely. Prolactin was apparently effective on the formation of end-buds. The present results provide the definite evidence that PL and GH are the primary important mammotropins in mice.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 発行日訂正内容: 訂正後 : 19710125

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) DISCHE, Z. (1930) Mikrochemie, 8: 4.
2) FLUX, D. S. (1957) J. Endocr., 15: 266-272.
3) Idem (1958) Ibid., 17: 300-306.
4) HADFIELD, G. (1957) Lancet, 272: 1058-1061.
5) HADFIELD, G. and S. YOUNG (1958) Br. J. Surg., 46: 265-273.
6) MEITES, J. (1965) Endocrinology, 76: 1220-1223.
7) Idem (1966) In "Neuroendocrinology", Vol. I, MARTINI, L. and W. F. GANONG eds. 669-707. Academic Press, New York and London.
8) MIXNER, J. P. and C. W. TURNER (1943) Endocrinology, 30: 706-710.
9) MIZUNO, H., K. IIDA and M. NAITO (1955) Endocrinol. Japon. 2: 163-169.
10) MIZUNO, H. and M. NAITO (1956) Ibid., 3: 227-230.
12) NANDI, S. (1958 a) Science, 128: 772-774.
13) Idem (1958 b) J. Nat. Cancer Inst., 21: 1039-1063.
14) Idem (1959) Univ. Calif. Publ. Zool., 65: 1-128.
15) SCHNEIDER, W. C. (1945) J. Biol. Chem., 161:293-303.
16) TALWALKER, P. K. and J. MEITES (1961) Proc. Soc. exp. Biol. Med., 107: 880-883.

Right : 1) DISCHE, Z. (1930) Mikrochemie, 8: 4.
2) FLUX, D. S. (1957) J. Endocr., 15: 266-272.
3) FLUX, D. S. (1958) J. Endocr., 17: 300-306.
4) HADFIELD, G. (1957) Lancet, 272: 1058-1061.
5) HADFIELD, G. and S. YOUNG (1958) Br. J. Surg., 46: 265-273.
6) MEITES, J. (1965) Endocrinology, 76: 1220-1223.
7) MEITES, J. (1966) In "Neuroendocrinology", Vol. I, MARTINI, L. and W. F. GANONG eds. 669-707. Academic Press, New York and London.
8) MIXNER, J. P. and C. W. TURNER (1943) Endocrinology, 30: 706-710.
9) MIZUNO, H., K. IIDA and M. NAITO (1955) Endocrinol. Japon. 2: 163-169.
10) MIZUNO, H. and M. NAITO (1956) Endocrinol. Japon., 3: 227-230.
12) NANDI, S. (1958 a) Science, 128: 772-774.
13) NANDI, S. (1958 b) J. Nat. Cancer Inst., 21: 1039-1063.
14) NANDI, S. (1959) Univ. Calif. Publ. Zool., 65: 1-128.
15) SCHNEIDER, W. C. (1945) J. Biol. Chem., 161:293-303.
16) TALWALKER, P. K. and J. MEITES (1961) Proc. Soc. exp. Biol. Med., 107: 880-883.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

マウス乳腺発育におけるPLおよびGHの重要性を検討するために,副腎•卵巣除去マウスに,除去した翌日から毎日,PL (0.5mg), GH (1.0mg)あるいはPL (0.5mg)+GH (1.0mg)を1日2回1週間投与したところ,各群とも,量的にも質的にも無処置対照群のレベルを維持し,PL+GHでは,無処置対照群よりもむしろ優れた結果を示した.一方,副腎•卵巣除去後一度乳腺を退行させてから1週間後に,これらホルモンを10日間投与し続けると,PL群では,量的には無処置対照群よりもやや劣る傾向があったが,end-budの形成には有効であった.また,GH群ではend-budの形成はあまり顕著ではなかったが,量的には充分無処置マウスのレベルにまで回復した.PL+GH群では,量的には無処置マウスのレベルにまで回復し,質的には,むしろ無処置対照群よりも優れていた.以上の結果,比較的大量のPLあるいはGHは,ステロイドホルモンのない状態でも乳腺を維持ないし発育させ得ることが明らかとなり,これらホルモンがmammotropinとして第一義的に重要な要因であると考えられた.

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