ブタ保存臓器からのミトコンドリアDNA精製とそのRFLP分析

坂上 正行; 田中 一栄; 鈴木 伸一; 坂井 活子; 向山 明孝

doi:10.2508/chikusan.62.125

坂上正行, 田中一栄, 鈴木伸一, 坂井活子, 向山明孝

著者情報

ジャーナルフリー

62 巻 (1991) 2 号 p. 125-134

DOI https://doi.org/10.2508/chikusan.62.125

詳細

公開日
公開日: 2008/03/10 [早期公開] 公開日: - 受付日: 1990/06/26 改訂日: - 受理日: -
訂正情報
訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) FRANCISCO, J.F., G.G. BROWN and M.V. SIMPSON, Further studies on type A and B rat mtDNAs : cleavage maps and evidence for cytoplasmic inheritance in mammals. Plasmid, 2: 426-436. 1979.
2) GILES, R.E., H. BLANC, H.M. CANN and D.G. WALLACE, Maternal inheritance of human mitochondrial DNA. Proc. Natl. Acad. Sci., 77: 6715-6719. 1980.
3) HAUSWIRTH, W.W. and P.J. LAIPIS, Mitochondrial DNA polymorphism in a maternal lineage of Holstein cows. Proc. Natl. Acad. Sci., 79: 4686-4690. 1982.
4) HAYASHI, J., H. YONEKAWA, O. GOTOH, J. WATANABE and Y. TAGASHIRA, Strictly maternal inheritance of rat mitochondrial DNA. Biochem. Biophys. Res. Commun., 83: 1032-1038. 1978.
5) HORAI, S., T. GOJOBORI and E. MATSUNAGA, Mitochondrial DNA polymorphism in Japanese I. Analysis with restriction enzymes of six base pair recognition. Hum. Genet., 68: 324-332, 1984.
6) HUTCHINSON, C.A., J.E. NEWBOLD, S.S. POTTER and M.H. EDGELL, Maternal inheritance of mammalian mitochondrial DNA. Nature, 251: 536-538. 1974.
8) NARITA, A and K. ISHIDA, Age-related change in the number of mitochondria in mouse oocytes. Jpn. J. Zootech. Sci., 61 30-38. 1990.
9) NASS, M.M.K., Mitochondrial DNA : Advances, Problems, and Goals; Studies of size and structure of mitochondrial DNA relate to biogenesis and function of this organelle. Science, 165: 25-35. 1969.
10) POTTER, S.S., J.E. NEWBOLD, C.A. HUTCHISON III and M.H. EDGELL, Specific cleavage analysis of mammalian mitochondrial DNA. Proc. Natl. Acad. Sci., 72: 4496-4500. 1975.
11) POWELL, J.R. and M.C. ZUPIGA, A simplified procedure for studying mtDNA polymorphisms. Biochem. Genet., 21: 1051-1055. 1983.
12) SCHNEIDER, W.C. and E.L. KUFF, The isolation and some properties of rat liver mitochondrial deoxyribonucleic acid. Proc. Natl. Acad. Sci., 54: 1650-1658. 1965.
13) WATANABE, T., Y. HAYASHI, N. OGASAWARA and T. TOMITA, Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease cleavage patterns. Biochem. Genet., 23: 105-113. 1985.
14) WATANABE, T., Y. HAYASHI, J. KIMURA, Y. YASUDA, N. SAITOU, T. TOMITA and N. OGASAWARA, Pig mitochondrial DNA: Polymorphism, restriction map orientation, and sequence data. Biochem. Genet., 24: 385-396. 1986.

Right : 1) FRANCISCO, J.F., G.G. BROWN and M.V. SIMPSON, Further studies on type A and B rat mtDNAs : cleavage maps and evidence for cytoplasmic inheritance in mammals. Plasmid, 2: 426-436. 1979.
2) GILES, R.E., H. BLANC, H.M. CANN and D.G. WALLACE, Maternal inheritance of human mitochondrial DNA. Proc. Natl. Acad. Sci., 77: 6715-6719. 1980.
3) HAUSWIRTH, W.W. and P.J. LAIPIS, Mitochondrial DNA polymorphism in a maternal lineage of Holstein cows. Proc. Natl. Acad. Sci., 79: 4686-4690. 1982.
4) HAYASHI, J., H. YONEKAWA, O. GOTOH, J. WATANABE and Y. TAGASHIRA, Strictly maternal inheritance of rat mitochondrial DNA. Biochem. Biophys. Res. Commun., 83: 1032-1038. 1978.
5) HORAI, S., T. GOJOBORI and E. MATSUNAGA, Mitochondrial DNA polymorphism in Japanese I. Analysis with restriction enzymes of six base pair recognition. Hum. Genet., 68: 324-332, 1984.
6) HUTCHINSON, C.A., J.E. NEWBOLD, S.S. POTTER and M.H. EDGELL, Maternal inheritance of mammalian mitochondrial DNA. Nature, 251: 536-538. 1974.
8) NARITA, A and K. ISHIDA, Age-related change in the number of mitochondria in mouse oocytes. Jpn. J. Zootech. Sci., 61 30-38. 1990.
9) NASS, M.M.K., Mitochondrial DNA : Advances, Problems, and Goals; Studies of size and structure of mitochondrial DNA relate to biogenesis and function of this organelle. Science, 165: 25-35. 1969.
10) POTTER, S.S., J.E. NEWBOLD, C.A. HUTCHISON III and M.H. EDGELL, Specific cleavage analysis of mammalian mitochondrial DNA. Proc. Natl. Acad. Sci., 72: 4496-4500. 1975.
11) POWELL, J.R. and M.C. ZUPIGA, A simplified procedure for studying mtDNA polymorphisms. Biochem. Genet., 21: 1051-1055. 1983.
12) SCHNEIDER, W.C. and E.L. KUFF, The isolation and some properties of rat liver mitochondrial deoxyribonucleic acid. Proc. Natl. Acad. Sci., 54: 1650-1658. 1965.
13) WATANABE, T., Y. HAYASHI, N. OGASAWARA and T. TOMITA, Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease cleavage patterns. Biochem. Genet., 23: 105-113. 1985.
14) WATANABE, T., Y. HAYASHI, J. KIMURA, Y. YASUDA, N. SAITOU, T. TOMITA and N. OGASAWARA, Pig mitochondrial DNA: Polymorphism, restriction map orientation, and sequence data. Biochem. Genet., 24: 385-396. 1986.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) FRANCISCO, J.F., G.G. BROWN and M.V. SIMPSON, Further studies on type A and B rat mtDNAs : cleavage maps and evidence for cytoplasmic inheritance in mammals. Plasmid, 2: 426-436. 1979.
2) GILES, R.E., H. BLANC, H.M. CANN and D.G. WALLACE, Maternal inheritance of human mitochondrial DNA. Proc. Natl. Acad. Sci., 77: 6715-6719. 1980.
3) HAUSWIRTH, W.W. and P.J. LAIPIS, Mitochondrial DNA polymorphism in a maternal lineage of Holstein cows. Proc. Natl. Acad. Sci., 79: 4686-4690. 1982.
4) HAYASHI, J., H. YONEKAWA, O. GOTOH, J. WATANABE and Y. TAGASHIRA, Strictly maternal inheritance of rat mitochondrial DNA. Biochem. Biophys. Res. Commun., 83: 1032-1038. 1978.
5) HORAI, S., T. GOJOBORI and E. MATSUNAGA, Mitochondrial DNA polymorphism in Japanese I. Analysis with restriction enzymes of six base pair recognition. Hum. Genet., 68: 324-332, 1984.
6) HUTCHINSON, C.A., J.E. NEWBOLD, S.S. POTTER and M.H. EDGELL, Maternal inheritance of mammalian mitochondrial DNA. Nature, 251: 536-538. 1974.
8) NARITA, A and K. ISHIDA, Age-related change in the number of mitochondria in mouse oocytes. Jpn. J. Zootech. Sci., 61 30-38. 1990.
9) NASS, M.M.K., Mitochondrial DNA : Advances, Problems, and Goals; Studies of size and structure of mitochondrial DNA relate to biogenesis and function of this organelle. Science, 165: 25-35. 1969.
10) POTTER, S.S., J.E. NEWBOLD, C.A. HUTCHISON III and M.H. EDGELL, Specific cleavage analysis of mammalian mitochondrial DNA. Proc. Natl. Acad. Sci., 72: 4496-4500. 1975.
11) POWELL, J.R. and M.C. ZUPIGA, A simplified procedure for studying mtDNA polymorphisms. Biochem. Genet., 21: 1051-1055. 1983.
12) SCHNEIDER, W.C. and E.L. KUFF, The isolation and some properties of rat liver mitochondrial deoxyribonucleic acid. Proc. Natl. Acad. Sci., 54: 1650-1658. 1965.
13) WATANABE, T., Y. HAYASHI, N. OGASAWARA and T. TOMITA, Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease cleavage patterns. Biochem. Genet., 23: 105-113. 1985.
14) WATANABE, T., Y. HAYASHI, J. KIMURA, Y. YASUDA, N. SAITOU, T. TOMITA and N. OGASAWARA, Pig mitochondrial DNA: Polymorphism, restriction map orientation, and sequence data. Biochem. Genet., 24: 385-396. 1986.

Right : 1) FRANCISCO, J.F., G.G. BROWN and M.V. SIMPSON, Further studies on type A and B rat mtDNAs : cleavage maps and evidence for cytoplasmic inheritance in mammals. Plasmid, 2: 426-436. 1979.
2) GILES, R.E., H. BLANC, H.M. CANN and D.G. WALLACE, Maternal inheritance of human mitochondrial DNA. Proc. Natl. Acad. Sci., 77: 6715-6719. 1980.
3) HAUSWIRTH, W.W. and P.J. LAIPIS, Mitochondrial DNA polymorphism in a maternal lineage of Holstein cows. Proc. Natl. Acad. Sci., 79: 4686-4690. 1982.
4) HAYASHI, J., H. YONEKAWA, O. GOTOH, J. WATANABE and Y. TAGASHIRA, Strictly maternal inheritance of rat mitochondrial DNA. Biochem. Biophys. Res. Commun., 83: 1032-1038. 1978.
5) HORAI, S., T. GOJOBORI and E. MATSUNAGA, Mitochondrial DNA polymorphism in Japanese I. Analysis with restriction enzymes of six base pair recognition. Hum. Genet., 68: 324-332, 1984.
6) HUTCHINSON, C.A., J.E. NEWBOLD, S.S. POTTER and M.H. EDGELL, Maternal inheritance of mammalian mitochondrial DNA. Nature, 251: 536-538. 1974.
8) NARITA, A and K. ISHIDA, Age-related change in the number of mitochondria in mouse oocytes. Jpn. J. Zootech. Sci., 61 30-38. 1990.
9) NASS, M.M.K., Mitochondrial DNA : Advances, Problems, and Goals; Studies of size and structure of mitochondrial DNA relate to biogenesis and function of this organelle. Science, 165: 25-35. 1969.
10) POTTER, S.S., J.E. NEWBOLD, C.A. HUTCHISON III and M.H. EDGELL, Specific cleavage analysis of mammalian mitochondrial DNA. Proc. Natl. Acad. Sci., 72: 4496-4500. 1975.
11) POWELL, J.R. and M.C. ZUPIGA, A simplified procedure for studying mtDNA polymorphisms. Biochem. Genet., 21: 1051-1055. 1983.
12) SCHNEIDER, W.C. and E.L. KUFF, The isolation and some properties of rat liver mitochondrial deoxyribonucleic acid. Proc. Natl. Acad. Sci., 54: 1650-1658. 1965.
13) WATANABE, T., Y. HAYASHI, N. OGASAWARA and T. TOMITA, Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease cleavage patterns. Biochem. Genet., 23: 105-113. 1985.
14) WATANABE, T., Y. HAYASHI, J. KIMURA, Y. YASUDA, N. SAITOU, T. TOMITA and N. OGASAWARA, Pig mitochondrial DNA: Polymorphism, restriction map orientation, and sequence data. Biochem. Genet., 24: 385-396. 1986.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

低温保存したブタの臓器からミトコンドリアDNA(mtDNA)を精製し,その精製mtDNAを用いたRFLP分析を行なった.ブタの肝臓,精巣,胎盤の各臓器を各種温度条件下で保存し,これらの臓器からmtDNAを定法に従って精製したところ,採取後21日目まで-20°Cないし-80°C下で保存された各臓器からは,いずれもRFLP分析に十分な純度のmtDNAが精製され,その電気泳動像は,ocDNAとcccDNA領域を示す2本のバンドが検出された.低温保存下の物理的な切断によるRFLP分析への影響は認められなかった.肝臓10g当たりからのmtDNAの収量は約300μgであり,RFLP分析に必要な電気泳動用試料として十分な収量であった.また,各臓器におけるmtDNAの収量は,肝臓>精巣>胎盤の順に多かったが,血液からの收量は極めて少なく,RFLPの検出には不適当と思われた.なお,低温保存臓器から精製されたmtDNAの電気泳動像は,4°Cにて21日間保存された各臓器についてはバンドが検出されなかった.以上の結果にもとづき,保存した肝臓を対象として各ブタ品種が有するmtDNA型を調査したところ,ランドレース種,ハンプシャー種においていずれも同一型が検出された.また,mtDNAのRFLPは,大ヨークシャー種,中ヨークシャー種に検出されたA型およびB型に見られるように.ブタの複雑な品種成立の過程をよく反映する指標となることが示された.さらに,三元交雑種LWDの持つmtDNA型の調査によって,ブタのmtDNAが母性遺伝を示すことが確認された.

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