Circulation Journal
Online ISSN : 1347-4820
Print ISSN : 1346-9843
ISSN-L : 1346-9843
Effects of Salt Loading on Plasma Osteoprotegerin Levels and Protective Role of Potassium Supplement in Normotensive Subjects
Fu-Qiang LiuSheng-Qiang LiuYong ZhangYang WangChao ChuDan WangShuo PanJun-Kui WangQi YuJian-Jun Mu
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Article ID: CJ-16-0756

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Abstract

Background: Excess dietary salt is strongly correlated with cardiovascular disease, morbidity, and mortality. Conversely, potassium likely elicits favorable effects on cardiovascular disorders. In epidemiological studies, increased plasma osteoprotegerin (OPG) concentrations are associated with atherosclerosis and vascular deaths. Our study was designed to examine the effects of salt intake and potassium supplementation on plasma OPG levels in normotensive subjects.

Methods and Results: The 18 normotensive subjects were selected from a rural community in China. They were sequentially maintained on low-salt diet for 7 days (3 g/day, NaCl), high-salt diet for 7 days (18 g/day), and high-salt diet with potassium supplementation for 7 days (18 g/day of NaCl+4.5 g/day of KCl). High-salt intake enhanced plasma OPG levels (252.7±13.9 vs. 293.4±16.1 pg/mL). This phenomenon was abolished through potassium supplementation (293.4±16.1 vs. 235.1±11.3 pg/mL). Further analyses revealed that the OPG concentration positively correlated with 24-h urinary sodium excretion (r=0.497, P<0.01). By contrast, OPG concentration negatively correlated with 24-h urinary potassium excretion (r=0.594, P<0.01).

Conclusions: Salt loading can enhance the production of circulating OPG. Potassium supplementation can reverse the effects of excessive OPG. Our study results may improve our understanding of the roles of salt and potassium in the risk of cardiovascular disorders.

Excess dietary salt induces adverse cardiovascular and renal effects, as demonstrated in numerous epidemiological, interventional, and experimental studies.13 Hypertension prevention studies have provided supporting evidence that modest reductions in salt intake promote 25% long-term reduction in the risk of cardiovascular events; thus, salt intake is implicated in cardiovascular function.4 Several mechanisms, including endothelial dysfunction, oxidative stress, inflammation, insulin resistance, and neurogenically mediated increase in peripheral resistance, contribute to the harmful effects of dietary salt.59 Potassium likely elicits favorable effects on cardiovascular morbidity and mortality.1012 Dietary potassium supplementation may prevent the salt loading-induced elevation of blood pressure (BP) by inhibiting the sympathetic nerve excitability, salt-induced insulin resistance, and oxygen-reactive free radical generation.

Osteoprotegerin (OPG), a soluble member of the tumor necrosis factor (TNF) receptor superfamily, acts as a decoy receptor of the receptor activator of nuclear factor κ-B ligand (RANKL) and the TNF-related apoptosis-inducing ligand (TRAIL); RANKL and TRAIL are 2 cytokines of the TNF family mainly involved in bone metabolism; they are also found in other tissues, such as vascular smooth muscle cells.13 OPG has been implicated in various inflammatory reactions and endothelial dysfunctions, which are also associated with diabetes mellitus and atherosclerosis.1416 Increased plasma OPG concentrations are also related to coronary artery disease frequently accompanied by atherosclerosis, stroke, diabetes mellitus, vascular death, and overall cardiovascular morbidity and mortality.1720

Data are still scarce in the literature with regard to the link between salt intake and OPG. Our study was designed to examine the effects of salt intake and potassium supplementation on plasma OPG levels in non-obese, normotensive subjects.

Methods

Subjects

A total of 18 male subjects with similar dietary preferences were recruited from a rural community in northern China. A brief medical questionnaire was administered. Subjects with a history of hypertension, obesity, liver or renal disease, or diabetes mellitus were excluded. Hypertension was defined as the mean systolic BP ≥140 mmHg and/or mean diastolic BP ≥90 mmHg. Obesity was defined as body mass index (BMI) ≥28 kg/m2. All subjects were nonsmokers. The study protocol was approved by the Ethics Committee of Xi’an Jiaotong University Medical School. Written informed consent was given by each subject. The procedures were performed in accordance with institutional guidelines.

Protocol

The protocol consisted of a series of investigations, including a 3-day baseline period during which clinical history and physical examination data (height, weight, and BP) were obtained. The subjects were given a normal salt diet, followed by a low-salt diet (51.3 mmol or 3 g of NaCl/day) for 7 days, high-salt diet (307.7 mmol or 18 g of NaCl/day) for 7 days, and high-salt diet with potassium supplementation (60 mmol or 4.5 g of KCl/day) for 7 days. In the baseline investigation period, each subject was given detailed dietary instructions to avoid table salt, cooking salt, high-sodium food, and nitrite/nitrate-rich food for the next 21 days. Meals were prepared in research kitchens and consumed onsite.

Biochemical Analyses

Blood glucose level was measured via glucose oxidase method. Serum lipids, including total cholesterol, triglycerides, and high-density lipoprotein cholesterol (HDL-C), were also determined. Blood samples for the measurement of fasting plasma OPG concentrations were collected in EDTA-aprotinin tubes and immediately placed in an ice bath. The tubes were centrifuged at 4℃, and plasma was collected and stored at −80℃ until analysis. OPG concentration was determined using a validated sandwich ELISA with an OPG-specific antibody (RayBiotech, Inc., Norcross, GA, USA). Five plasma samples for OPG were used to evaluate the intra- and interassay coefficients of variation, which ranged from 3.3% to 4.6% (mean, 3.9%) and from 5.7% to 7.2% (mean, 6.4%), respectively.

24-h Urinary Sodium and Potassium Determination

Urine samples (24 h) were collected at baseline and on day 7 of each intervention period. The samples were kept frozen at −40℃ until analysis. The urinary concentrations of sodium and potassium were determined using ion-selective electrodes (Hitachi, Ltd., Tokyo, Japan). The 24-h urinary excretion of sodium and potassium was quantified by multiplying the sodium and potassium concentrations with the 24-h total urine volume.

Statistical Analysis

Data are presented as mean±SD. Differences between biochemical markers obtained with low- and high-salt intake were calculated through ANOVA with repeated measures. Age, sex, and BMI were adjusted through multivariable analysis. Calculations were performed using SPSS 16.0 for Windows (Chicago, IL, USA). Probability was assessed using a two-tailed P-value <0.05 to describe statistical significance.

Results

Profiles of the Study Subjects

All the enrolled subjects completed this interventional study. Their average age was 50.7±6.6 years, and systolic BP and diastolic BP were 117.2±9.2 and 72.4±6.2 mmHg, respectively (Table 1). The average 24-h urinary sodium excretion was 173.2±12.1 mmol/day, which corresponded to an intake of 8 g/day of salt. The average daily urinary potassium excretion was 45.3±5.3 mmol/day.

Table 1. Baseline Demographic and Clinical Characteristics of the Normotensive Study Subjects
Parameter Value
Mean age (years) 50.7±6.6
Sex (M/F) 9/9
BMI (kg/m2) 21.8±0.6
SBP (mmHg) 117.2±9.2
DBP (mmHg) 72.4±6.2
Fasting glucose (mmol/L) 3.92±0.91
Hemoglobin (g/L) 134.5±2.7
Total cholesterol (mmol/L) 4.05±1.0
Triglycerides (mmol/L) 1.36±0.89
LDL-cholesterol (mmol/L) 2.24±0.83
HDL-cholesterol (mmol/L) 1.17±0.25
Serum creatinine (μmol/L) 56.98±9.21
Serum sodium (mmol/L) 142.03±1.09
Serum potassium (mmol/L) 3.94±0.33
Plasma OPG level (pg/mL) 265.1±11.3

BMI, body mass index; BP, blood pressure; DBP, diastolic BP; HDL, high-density lipoprotein; LDL, low-density lipoprotein; OPG, osteoprotegerin; SBP, systolic BP.

Effects of Dietary Intervention on BP and 24-h Urinary Sodium and Potassium Excretion

Table 2 shows the BP responses to the dietary interventions. The BP responses to the low-salt diet, high-salt diet, and high-salt diet with potassium supplement intervention did not change significantly. The 24-h sodium and potassium excretion in urine was calculated at the end of the intervention period to ensure compliance with the study protocol. Urinary sodium excretion was significantly decreased as the baseline was changed to the low-salt diet, but increased as the low-salt diet was changed to the high-salt diet (P<0.05; Table 2). Potassium supplementation increased urinary potassium excretion and slightly increased urinary sodium excretion. These results confirmed that the subjects complied with the dietary intervention protocol.

Table 2. BP (mmHg) and 24-h Urinary Sodium and Potassium Excretion (mmol/day) at Baseline and During Dietary Interventions
  SBP DBP 24-h Urinary Na+
(mmol/day)
24-h Urinary K+
(mmol/day)
Baseline 117.2±9.2 72.4±6.2 173.2±12.1* 45.3±5.3
Low-salt diet 119.3±9.5 73.1±4.6 99.6±3.7 38.1±3.1
High-salt diet 120.6±11.1 74.5±6.6 244.5±13.8* 41.6±2.8
High-salt diet+potassium supplement 117.4±10.9 73.2±6.5 259.1±4.7# 71.6±3.1

*P<0.05 vs. low-salt diet; #P<0.05 vs. high-salt diet. Abbreviations as in Table 1.

Effects of High Salt Intake and Potassium Supplementation on Fasting OPG Levels

There was no difference in plasma OPG levels between the baseline and the low-salt diet period (265.1±11.3 vs. 252.7±13.9 pg/mL; Figure 1). High-salt intake enhanced the plasma OPG levels (252.7±13.9 pg/mL vs. 293.4±16.1 pg/mL). The high-salt-diet-induced increase in plasma OPG was prevented by potassium supplementation (293.4±16.1 vs. 235.1±11.3 pg/mL). Further analyses revealed that the OPG level correlated with the 24-h urinary sodium excretion in the low- and high-salt dietary intervention periods (r=0.497, P<0.01; Figure 2). The plasma OPG level negatively correlated with the 24-h urinary potassium excretion in the high-salt diet alone and the high-salt diet with potassium supplementation intervention periods (r=0.594, P<0.01; Figure 3). Moreover, the OPG level positively correlated with the 24-h urinary sodium excretion Na/K ratio (r=0.311, P=0.008; Figure 4).

Figure 1.

Effect of salt intake and potassium supplement on fasting osteoprotegerin levels in all study subjects.

Figure 2.

Correlation between plasma osteoprotegerin levels and 24-h urinary sodium excretion (mmol/day) in all study subjects on a low-salt diet and on a high-salt diet.

Figure 3.

Correlation between plasma osteoprotegerin levels and 24-h urinary potassium excretion (mmol/day) in all study subjects on a high-salt diet and on a high-salt diet with potassium supplement.

Figure 4.

Correlation between plasma osteoprotegerin level and 24-h urinary Na/K ratio in all study subjects at baseline and in the diet intervention period.

Discussion

This study found that high-salt intake can stimulate OPG production. Conversely, potassium supplementation can prevent the effects of high-salt diet on plasma OPG levels. Moreover, the plasma OPG level positively correlated with the 24-h urinary Na/K ratio.

OPG is associated with the onset and progression of cardiovascular disease (CVD); this substance has been considered as a potential biomarker of cardiometabolic disorders.1721 Although OPG was initially identified as a bone reabsorption regulator, it is synthesized by mononuclear leukocytes and endothelial cells; OPG is also expressed in the central nervous system, thyroid, heart, liver, lung, testes, ovaries, pancreas, adrenals, and kidneys.16 OPG regulates several important organ systems by inhibiting the interaction between RANKL and its receptor RANK. The OPG/RANKL/RANK system is involved in the pathogenesis of endothelial dysfunction, vascular inflammation, and vascular calcification. In 2001, the first study reporting a positive association of OPG with all-cause and CVD death and incident diabetes was published.22 In the prospective, population-based Bruneck Study, Kiechl and colleagues19 observed a significant association of OPG with the 10-year incidence of CVD defined as myocardial infarction, stroke, transient ischemic attack, or peripheral artery disease and vascular death associated with myocardial infarction, stroke, ruptured aneurysm, or sudden cardiac death; OPG is also related to the initiation and progression of carotid atherosclerosis.19 Increased OPG levels have been observed in patients with unstable angina, acute myocardial infarction, or significant coronary artery stenosis.19,20,23 Lewis et al conducted a prospective study of elderly women and demonstrated that increased circulating OPG levels and renal dysfunction can predict 15-year CVD and all-cause mortality rates.24 Furthermore, OPG is associated with atherosclerosis and diabetic complications, such as neuropathy, nephropathy, or retinopathy.17,21,25 However, studies have yet to determine whether OPG is causally related to atherosclerotic disease progression or is implicated as a modulatory response to subclinical CVD. In endothelial cells, OPG acts as a survival and antiapoptotic factor. It protects endothelial cells from apoptosis in vitro and promotes neovascularization in vivo. OPG enhances endothelial cell proliferation in microvessels.26 Therefore, high circulating levels of OPG may indicate a compensatory mechanism by which injuries in blood vessel walls induce the release of OPG from the inner blood vessel wall to the circulation. OPG also plays a pathogenic role in atherogenesis, inducing the expression of ICAM-1, VCAM-1, and E-selectin on endothelial cells and consequently promoting leukocyte adhesion, which is an early step in endothelial cell dysfunction. At high concentrations, OPG can stimulate metalloprotease activity in vascular smooth muscle cells.15,27,28 Therefore, further in vitro and in vivo studies should be performed to establish the interaction between OPG and endothelial dysfunction or inflammation.

Although the mechanisms underlying the association of high-salt intake and cardiovascular events remain unresolved, studies have confirmed that endothelial dysfunction and inflammation play crucial roles in this phenomenon. Li et al demonstrated that a 5 mmol/L increase in salt concentration causes a 25% decrease in nitric oxide (NO) synthase activity in vivo.7,8 High-salt diet impairs endothelium-dependent relaxation by decreasing NO levels and increasing superoxide production in rats.5 Our previous dietary intervention studies also showed that a high-salt diet can impair endothelial function and facilitate inflammation in normotensive subjects, especially in salt-sensitive patients.29,30 Our present study demonstrated that salt loading significantly enhanced the plasma OPG level and that the 24-h urinary sodium excretion positively associated with the OPG level in humans. This study provided a novel finding that explains the link between sodium intake and CVD incidence. However, further studies should be performed to confirm the role of OPG in adverse salt-induced outcomes.

Our work also provides novel insights into the effects of potassium supplementation on the influence of high-salt diet on OPG levels. In particular, potassium supplementation can reverse the effects of high-salt diet on OPG levels. However, the molecular mechanisms modulating the serum OPG level in response to potassium supplementation remain elusive. Our previous studies demonstrated that potassium supplementation in normotensive subjects may influence NOx synthesis by inhibiting asymmetrical dimethylarginine production.31 Wyss et al showed that potassium directly restores the vasorelaxation of resistance arterioles in non-hypertensive DOCA/salt-fed mice, possibly via its antioxidant effect.32 Overall, potassium supplementation may counteract the salt-induced OPG increase by inhibiting oxidative stress and protecting endothelial functions. Thus, this phenomenon contributes to a reduction in CVD risk.

Study Limitations

Our observation cannot be considered as a general finding to be applied to other populations, because of the small sample size. Further studies should be conducted to validate our findings in a larger and more diverse sample. The mechanisms by which salt loading affects plasma OPG and the protective effect of potassium supplementation need further investigation.

In conclusion, our human intervention study found that salt loading could increase the amount of circulating OPG. Potassium supplementation could reverse the effects of excessive OPG. These findings may enhance our understanding of the roles of salt and potassium in CVD morbidity and mortality. Our study may also have potential clinical and public health implications.

Grants

This study was supported by grant 81200512 (to F.-Q.L.) from the Natural Science Foundation of China.

References
 
© 2016 THE JAPANESE CIRCULATION SOCIETY
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