Congress of the Japanese Physical Therapy Association
[volume title in Japanese]
Session ID : E-R-1-2
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English Session
Diencephalon Plays an Active Role in Respiratory Drive
Isato FukushiYosuke KonoShigefumi YokotaKotaro TakedaShuntaro OkazakiItaru YazawaHiroshi OnimaruYasumasa Okada
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The diencephalon is one of the brain regions where stroke occurs frequently. Because patients with stroke often present respiratory disorders and it has been suggested that the diencephalon plays a role in modulation of respiration, it would be necessary to elucidate how the diencephalon modulate respiration. Here, we aimed to address this issue.


We examined respiratory frequency in the isolated diencephalon-lower brainstem-spinal cord preparations of newborn rats (n=11, P0-P1), before and after transection of the diencephalon. Briefly, the diencephalon, lower brainstem and spinal cord were together isolated from neonatal rats under isoflurane anesthesia. The preparation was fixed in a recording chamber and continuously superfused with oxygenated (95% O2, 5% CO2) artificial cerebrospinal fluid at 26 °C ‒ 28 °C. Neural respiratory output was recorded from ventral roots of the 4th cervical spinal cord (C4). The respiratory frequency was obtained as the frequency of the C4 burst activity.


Ablation of the diencephalon decreased respiratory frequency. The paired t-test was carried out to examine significant differences of the respiratory frequency. Respiratory frequency was decreased after ablation with statistical significance (p = 0.007).


We conclude that the diencephalon plays an active role in facilitation of respiration. Further studies are needed to identify the diencephalic region that augments respiratory output.


[Ethical consideration]

This study was conducted with the approval of the Animal Experiment Ethics Committee of Murayama Medical Center.

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