1994 Volume 23 Issue 1 Pages 69-72
Chlorophyllase was purified 13300-fold from a crude butanol extract of Chlorella regularis. The enzyme clearly recognizes the C132 stereochemistry of chlorophyllous pigments in that it hydrolyzes the a and b (132R) forms but not the a′ and b′ (132S) forms. No enzyme was detected in vivo that specifically hydrolyzes chlorophyll a′.
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