Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
Purification and Substrate Specificity of Chlorophyllase from Chlorella regularis
Yoshitaka NishiyamaMasaya KitamuraSayuri TamuraTadashi Watanabe
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1994 Volume 23 Issue 1 Pages 69-72

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Abstract

Chlorophyllase was purified 13300-fold from a crude butanol extract of Chlorella regularis. The enzyme clearly recognizes the C132 stereochemistry of chlorophyllous pigments in that it hydrolyzes the a and b (132R) forms but not the a′ and b′ (132S) forms. No enzyme was detected in vivo that specifically hydrolyzes chlorophyll a′.

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© 1994 The Chemical Society of Japan
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