Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
A Facile Transgalactosylation catalyzed by a Lipid-Coated β-D-Galactosidase in the Water-Organic Two Phases
Toshiaki MoriSanae FujitaYoshio Okahata
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1997 Volume 26 Issue 1 Pages 73-74

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Abstract

A lipid-coated β-D-galactosidase could act as an efficient catalyst for transgalactosylation in the water-organic two phases: both the hydrophobic lipid-coated enzyme and alcohols were solubilized in isopropyl ether and mixed with an aqueous solution of lactose (a galactosyl donor). When a native β-D-galactosidase was employed for the same reaction, neither the transgalactosylation nor the hydrolysis reaction proceeded due to the deactivation of the enzyme at the interface.

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© 1997 The Chemical Society of Japan
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