Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
Letter
Photochemical Immobilization of Protein on Inner Wall of Microchannel
Hizuru NakajimaSatomi IshinoHironori MasudaTakuya ShimosakaTatsuro NakagamaToshiyuki HoboKatsumi Uchiyama
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2005 Volume 34 Issue 3 Pages 358-359

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Abstract

A novel strategy for patterning several kinds of proteins in a specific position of a microchannel is presented. Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) are covalently immobilized on a polystyrene microchannel surface through a photoreactive crosslinker with an azide group at one end and a succinimide group at the other by UV irradiation. The activities of HRP and ALP are evaluated using Amplex Red and Fluorescein diphosphate, respectively. The fluorescence has been observed only at the enzyme-immobilized position.

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© 2005 The Chemical Society of Japan
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