Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
Letter
On-chip Capillary Electrophoresis Fractionation of DNA Construct for Cell-free Protein Expression
Takahiko NojimaShohei KanedaTeruo Fujii
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2007 Volume 36 Issue 11 Pages 1346-1347

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Abstract

An on-chip capillary electrophoresis-based DNA collection was applied to isolate desired DNA construct from a reaction mixture of DNA-assembly instead of traditional living cell-based screening. The isolated construct was amplified with polymerase chain reaction and subjected to a coupled cell-free transcription/translation system as a template, and the template-dependent protein expression was observed. It was demonstrated that total process of standard recombinant DNA technology was performed in a cell-free condition.

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© 2007 The Chemical Society of Japan
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