Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
Letter
In-cell Viscosity Measurement Using a Fluorescence Up-conversion Microscope
Tatsuya FujinoKouji HirotaKunihiro OhtaTahei Tahara
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2008 Volume 37 Issue 12 Pages 1240-1241

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Abstract

Using a femtosecond fluorescence up-conversion microscope, viscosity values in yeast cells (budding, wine, and fission) were estimated based on ultrafast fluorescence dynamics of a fluorescent probe dye, Malachite Green. The decay time of the S1 state of Malachite Green in cytoplasm was determined to be 2.2–2.4 ps at room temperature, which corresponded to the viscosity value of 5–6 cP. Nondestructive estimation of viscosity value in cells (in-cell viscosity) was carried out.

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© 2008 The Chemical Society of Japan
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