Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
Letter
Cell-free Protein Synthesis Conducted by Template DNA with Repetitive Sequence
Takahiko NojimaHiroshi KimuraTeruo Fujii
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2008 Volume 37 Issue 6 Pages 648-649

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Abstract

DNA with repetitive sequence of green fluorescent protein (GFP) was prepared by rolling circle amplification with φ29 DNA polymerase from a bacterial expression vector carrying a GFP gene. The prepared DNA was subjected to a cell-free coupled transcription–translation system and green fluorescence was observed, which shows that the template with repetitive sequence properly functioned in the reaction. The efficiency of the reaction conducted by the template with repetitive sequence was higher than those by linear template coding single gene or by circular plasmid.

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© 2008 The Chemical Society of Japan
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