2010 Volume 39 Issue 11 Pages 1180-1181
A novel artificial antigen (AA) for Ni2+ was prepared by designed route, which coupled diethylenetriamine-N,N,N′,N′′,N′′-pentaacetic dianhydride (DTPAA) with bovine serum albumin (BSA) and human serum albumin (HSA), and then chelated the DTPAA–BSA/HSA with Ni2+. The AA was characterized by bicinchoninic acid (BCA) protein assay, atomic absorption spectroscopy (AAS), ultraviolet (UV) spectrum, fluorescence spectrum, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and 2,4,6-trinitrobenzenesulfonic acid assay (TNBS). The consistent results showed that the synthetic route different from the traditional method was effective, and the AA was synthesized successfully.
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