Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
Letter
Synthesis and Characterization of Artificial Antigens for Nickel
Ming GuoXiaoqing ChePing YangJinyu ShenJiayun Yao
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2010 Volume 39 Issue 11 Pages 1180-1181

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Abstract

A novel artificial antigen (AA) for Ni2+ was prepared by designed route, which coupled diethylenetriamine-N,N,N′,N′′,N′′-pentaacetic dianhydride (DTPAA) with bovine serum albumin (BSA) and human serum albumin (HSA), and then chelated the DTPAA–BSA/HSA with Ni2+. The AA was characterized by bicinchoninic acid (BCA) protein assay, atomic absorption spectroscopy (AAS), ultraviolet (UV) spectrum, fluorescence spectrum, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and 2,4,6-trinitrobenzenesulfonic acid assay (TNBS). The consistent results showed that the synthetic route different from the traditional method was effective, and the AA was synthesized successfully.

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© 2010 The Chemical Society of Japan
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