2013 Volume 42 Issue 10 Pages 1168-1169
A novel heparan sulfate disaccharide fluoride derivative of GlcN(SO3Na)α(1 → 4)GlcAβ-F (1) was designed as a probe for detection of heparanase activity. Compound 1 was successfully prepared via 10-step chemical reactions. Human heparanase-1 effectively recognized 1, which induced liberation of the fluoride ion by hydrolysis via enzyme catalysis. The hydrolysis reaction was easily monitored by 1H NMR measurements, in which the signal from H-1 gradually disappeared.
This article cannot obtain the latest cited-by information.