Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
Letter
A Continuous Fluorometric Assay for Trypsin Based on Melittin and the Noncovalent-binding-induced Pyrene Excimer
Na XuYue LiHong-Wei LiYuqing Wu
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2013 Volume 42 Issue 12 Pages 1528-1530

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Abstract

A continuous fluorometric assay system based on melittin and a pyrene derivative 1 for trypsin and its inhibitor screening is successfully developed by taking advantage of the noncovalent-binding-induced pyrene excimer. The 1–melittin assembly and its disassembly after further addition of trypsin are confirmed by the fluorescence changes at 475 nm. Such a system can be applied to other proteases of melittin, and essentially, the concept based on the noncovalent-binding-induced continuous fluorometric assay can be extended to other biochemical analyses.

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© 2013 The Chemical Society of Japan
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