Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
Letter
Enhancing Mechanism of Gene Transfection by Heat Shock
Misato TakizakiShun-ichiro MuranakaAung Thu HaineShigenori TokunagaShigeru MorimuraTakuro Niidome
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2017 Volume 46 Issue 8 Pages 1158-1160

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Abstract

Gene transfection can be enhanced by heat treatment. In this study, we examined the enhancement mechanism. Heat treatment before transfection showed higher enhancement than during or after the transfection. The heat treatment enhanced the total uptake of plasmid DNA, and increased caveolar endocytosis. As caveolar endocytosis is advantageous for escaping from lysosomal digestion, the amount of intact DNA may increase, resulting in enhanced gene expression.

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© 2017 The Chemical Society of Japan
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