Chemistry Letters
Online ISSN : 1348-0715
Print ISSN : 0366-7022
ISSN-L : 0366-7022
CATALYTIC ASSAY OF L-LACTATE OR PYRUVATE WITH AN ENZYME ELECTRODE BASED ON IMMOBILIZED LACTATE OXIDASE AND LACTATE DEHYDROGENASE
Fumio MizutaniYukio ShimuraKeishiro Tsuda
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JOURNALS FREE ACCESS

1984 Volume 13 Issue 2 Pages 199-202

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Abstract

An enzyme electrode using immobilized lactate oxidase and lactate dehydrogenase is prepared for the catalytic assay of L-lactate or pyruvate: the rate of the circulating enzymatic reactions between L-lactate and pyruvate is monitored by an oxygen electrode. Either of the substrates is determined in the concentration range 0.08–8 μM.

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