1975 Volume 4 Issue 6 Pages 547-550
From circular dichroic data, by utilyzing the method developed by Ang for determining the overlapping pK values, basic ionization constants have been evaluated for guanylyl-3′,5′-guanosine. This work establishes the first instance of the determination of the apparent ionization constants for the two protonation steps involved in homodinucleotides. A change of the circular dichroism with pH is convincing evidence for the existence of intermediate stacked monoprotonated species.
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