Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
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Direct Evidence for the Plant Origin of Brazilian Propolis by the Observation of Honeybee Behavior and Phytochemical Analysis
Shigenori KumazawaMasahiro YonedaIkoi ShibataJun KanaedaTomoko HamasakaTsutomu Nakayama
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2003 年 51 巻 6 号 p. 740-742

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To identify the plant origin of Brazilian propolis directly, we observed the behavior of honeybees in Minas Gerais State of Brazil. Honeybee workers bit and chewed leaves of the plant, Baccharis dracunculifolia, packed the material into their pollen basket, brought it back to their nest, and used it as propolis. We collected the leaves of B. dracunculifolia and propolis, and compared their constituents by liquid chromatography-mass spectrometry (LC/MS) analysis. There was no difference between the chemical constituents of the ethanol extracts of B. dracunculifolia and those of propolis. This indicates directly that the plant origin of Brazilian propolis is B. dracunculifolia.

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© 2003 The Pharmaceutical Society of Japan
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