Simple Synthesis of Deuterium and ¹³C Labeled Trifluoromethyl Phenyldiazirine Derivatives as Stable Isotope Tags for Mass Spectrometry

Abstract

The synthesis of trifluoromethyl diazirine with a stable isotope tag is reported. We found that both Friedel–Crafts acylation and reduction of aryl carbonyl to methylene, using commercially available stable-isotope reagents, were utilized for the synthesis of diazirinyl fatty acid derivatives. The stable isotope labeled diazirine may be valuable for identifying binding sites by mass spectrometry.