Quantitative Analysis of Marker Compounds in Angelica gigas, Angelica sinensis, and Angelica acutiloba by HPLC/DAD

Su Yang Jeong; Hye Mi Kim; Kyu Ha Lee; Kyu Yeob Kim; Dae Sun Huang; Jong Hwan Kim; Rack Seon Seong

doi:10.1248/cpb.c15-00081

Regular Articles

Quantitative Analysis of Marker Compounds in Angelica gigas, Angelica sinensis, and Angelica acutiloba by HPLC/DAD

Su Yang Jeong, Hye Mi Kim, Kyu Ha Lee, Kyu Yeob Kim, Dae Sun Huang, Jong Hwan Kim, Rack Seon Seong

Author information

Su Yang Jeong
Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety
Hye Mi Kim
Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety
Kyu Ha Lee
Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety
Kyu Yeob Kim
Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety
Dae Sun Huang
Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety
Jong Hwan Kim
Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety
Rack Seon Seong Corresponding author
Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety

Keywords: Angelica gigas, Angelica acutiloba, Angelica sinensis, HPLC/diode-array detector (DAD)

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Supplementary material

2015 Volume 63 Issue 7 Pages 504-511

DOI https://doi.org/10.1248/cpb.c15-00081

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Abstract

Although Danggui is the root of Angelica gigas NAKAI in the Korean Pharmacopoeia, it is determined that Danggui is also the root of Angelica sinensis (OLIV.) DIELS in China and Hong Kong, as well as the root of Angelica acutiloba KITAGAWA in Japan. Accordingly, we tried to develop an identification method using the main compounds in A. gigas, A. sinensis, and A. acutiloba through HPLC/diode-array detector (DAD). This method was fully validated for linearity, accuracy, precision, recovery, and robustness. Multivariate analysis was also implemented after pattern analysis and monitoring. As a result, each compound pattern of A. gigas, A. sinensis, and A. acutiloba was identified, making it possible to distinguish them from each other.

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