Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Special Collection of Papers: Notes
Identification and Heterologous Expression of the Topopyrone Nonaketide Synthase Gene from Phoma sp.
Nobuyuki KashiwaYutaka EbizukaIsao Fujii
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2016 Volume 64 Issue 7 Pages 947-951

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Abstract

Non-reducing iterative type I polyketide synthase genes, pnk1 and pnk2, were cloned from the fungus Phoma sp. BAUA2861, which produces the topoisomerase I inhibitors, topopyrones A to D. Heterologous expression of these polyketide synthase genes under the α-amylase promoter in Aspergillus oryzae was carried out to identify their functions. The pnk2 transformant produced topopyrones C, D, and haematommone. Therefore, the pnk2 gene was found to encode for the topopyrone nonaketide synthase.

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© 2016 The Pharmaceutical Society of Japan
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