抄録
Direct and simple methods for the preparations of 4-thiouridine 5'-phosphate (IVa), 6-thioinosine 5'-phosphate (IVb) and 6-thioguanosine 5'-phosphate (IVc) from the corresponding oxy-nucleotides are described. The 5'-ribonucleotides are converted to their 2', 3'-di-O-acetylated derivatives by a conventional acetylation procedure, followed by thiation with phosphorus pentasulfide to give crude thiated products. For the selective desulfurization, the crude products are treated with dicyclohexylcarbodiimide, followed by alkaline deacylation to give the desired "thio" nucleotides.
