Studies on Metabolism of 3-Deoxysteroids. X. Increased Activity of 3-Deoxyestrone Hydroxylases in Liver Microsomes of Rats pretreated with Phenobarbital and 3-Methylcholanthrene

Abstract

Effects of the pretreatment with phenobarbital or 3-methylcholanthrene on the hydroxylase activity of rats liver microsomes were examined with 3-deoxyestrone. The administration of phenobarbital stimulated the 6β-hydroxylase, while the 3-methyl-cholanthrene treatment increased the rate of aromatic hydroxylation selectively. The biotransformation products formed from 3-deoxyestrone with the hepatic microsomes could be characterized by the reverse isotope dilution method and the chromatographic behaviors.