Determination of Vanilmandelic Acid and Homovanillic Acid in Urine by Gas Chromatography

Abstract

Vanilmandelic acid and homovanillic acid in a small quantity of human urine were purified by adsorption on Amberlite XAD-4, added with 3-methoxy-4-hydroxyphenylethanol as an internal standard, and converted to trifluoroacetyl-hexafluoroisopropyl derivatives. Their quantities were determined by the use of a gas chromatograph equipped with a 2% OV-17 column and an electron capture detector.