Effect of phospholipid on fatty liver induced by orotic acid, carbon tetrachloride, or ethionine has been studied. Results are summarized as follows : 1. Orotic acid induced a marked accumulation of liver glycerides, and a decreased concentration of liver phospholipid and protein, and of serum beta-lipoprotein. These lesions were restored by administration of phospholipid. 2. In rats given carbon tetrachloride or ethionine, liver lipid accumulation and a decreased concentration of serum beta-lipoprotein were partially restored by administration of phospholipid, but a decreased protein content in the liver was unaffected. These results indicate that administration of phospholipid is effective in counteracting the depression of serum beta-lipoprotein and liver lipid accumulation by orotic acid, carbon tetrachloride, or ethionine. Phospholipid appears to have an important role in normal release of beta-lipoprotein.