Preparation of a Specific Antibody to Each One of Catecholamines and L-DOPA

AKIRA MIWA; MASANORI YOSHIOKA; AKIRA SHIRAHATA; YASUSHI NAKAGAWA; ZENZO TAMURA

doi:10.1248/cpb.24.1422

Chemical and Pharmaceutical Bulletin

Online ISSN : 1347-5223
Print ISSN : 0009-2363

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Volume 24 (1976) Issue 6
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Preparation of a Specific Antibody to Each One of Catecholamines and L-DOPA

AKIRA MIWA, MASANORI YOSHIOKA, AKIRA SHIRAHATA, YASUSHI NAKAGAWA, ZENZO TAMURA

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Volume 24 (1976) Issue 6 Pages 1422-1424

DOI https://doi.org/10.1248/cpb.24.1422

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Abstract

A method was developed for conjugation of catecholamines or L-DOPA with BSA leaving the catechol moiety and side chain intact. The specificity of the antibody, obtained by immunization of rabbits with the conjugate, was demonstrated by Ouchterlony method and by equilibrium dialysis.

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