Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Studies on the Pharmaceutical Quality Evaluation of Crude Drug Preparations used in Orient Medicine"Kampoo". II. Precipitation Reaction of Berberine and Glycyrrhizin in Aqueous Solution
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Keywords: glucuronic acid

1978 Volume 26 Issue 9 Pages 2624-2629


Precipitation reaction was found to occur when each 5×10-4 M aqueous solution of berberine (I) and glycyrrhizin (II) were blended. The molar ratio of I and II in the precipitate (III) was revealed to be 2 : 1 by observation of UV absorption at 251 and 350 nm. By comparison of the IR absorption near at 1715 and 1620 cm-1 of II, III, soyasaponin III and glycyrrhetinic acid after treated at various pH, it was revealed that the quartery base moiety of I bound on the glucouronic acid moieties of II ionically in compound III. The solubility of III in aqueous solution was 0.09% at pH 3.5, while it increased up to 1.9% at pH 6.8. The solubility of III was slightly decreased by addition of II in aqueous solution.

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