Isoflavonoids and the Other Constituents in Callus Tissues of Pueraria lobata

Abstract

Puerarin, daidzin, daidzein, genistein, coumesterol, methyl 2, 4-dihydroxybenzoate, a mixture of methyl p-hydroxybenzoate and methyl trans-p-hydroxycinnamate, phytosterols and aliphatic acid esters were isolated from the methanol extract of callus tissues, which were induced from the stem of Pueraria lobata OHWI. The main products at the final stage of callus culture were daidzin and puerarin in the suspension culture and daidzin in the static culture. The quantitative changes of daidzin, puerarin and daidzein during the growth of callus tissues were investigated.