Abstract
Female Fischer rats were sensitized to Bordetlla petussis vaccine mixed with Freund's complete adjuvant and challenged with pertussis organisms intrapleurally 7, 14 and 21 d later, then compared with non-sensitized rats. The thiobarbituric acid (TBA)-reactive substance and an acute phase reactant, ceruloplasmin, were monitored in the exudate or the serum. The retention volume of exudate in the non-sensitized rats scarcely increased after the intrapleural injection of pertussis organisms. On the other hand, in the sensitized rats, the retention volume of exudate increased rapidly after the challenge, and reached the peak value at 48 or 72 h, whereas the levels of TBA-reactive substance and ceruloplasmin in the exudate reached the maximum values at 24 h; a similar trend was found in the serum of the rats challengd 14 and 21 d later. It was found that the two parameters did not correlate to the retention of exudate and that lipid peroxidation is related to the acute phase response in this inflammatory model.
