Characterization of a Polysaccharide Having Activity on the Reticuloendothelial System from the Stolon of Glycyrrhiza globra var. glandulifera

Abstract

An acid polysaccharide, named glycyrrhizan GA, was isolated form the stolon of Glycyrrhiza glabra L. var. glandulifera REG.et HERD. It produced a single band on electrophoresis and a single peak on gel chromatography, and its molecular mass was estimated to be 85000. Glycyrrhizan GA is composed of L-arabinose : D-galactose : L-rhamnose : D-galacturonic acid : D-glucuronic acid in the molar ratio of 22 : 10 : 1 : 2 : 1, in addition to small amounts of O-acetyl groups. Part of the hexuronic acid residues exist as methyl esters. Methylation analysis, carbon-13 nuclear magnetic resonance and periodate oxidation studies indicated that its structural features include mainly α-arabino-β-3, 6-galactan type structural units. Glycyrrhizan GA showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test.