Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Preparation of Biologically Active and Site Specifically Radioiodinated Recombinant Human Insulin-like Growth Factor-I
Yasuhiro KOHAMAMasaru OKABEKazutake TSUJIKAWAHiroaki OKATetsuyuki TERAMOTOYuzo KAYAMORIMasaaki ITOHTsutomu MIMURA
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1992 Volume 40 Issue 3 Pages 808-810

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Abstract

Recombinant human insulin-like growth factor-I (rhIGF-I) was iodinated using a lactoperoxidase-catalyzed labeling method. The labeled products were separated into more than five fractions by ion-paired reverse-phase high performance liquid chromatography (HPLC). A fraction (peak 1), which showed the highest yield and radiactivity, was found to be biologically active in the BALB/c 3Ts cell proliferating system. The site of the iodination was investigated by S-pyridylethylation followed by trypsinization and separation with HPLC using reverse phase columns. From the amino acid analysis of the peaks which were radioactive, the iodination site of peak 1 was revealed to be Try-24 and Try-60. This is the first report of the biological activity of radioactive peptide hormone with a defined laveled site.

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© The Pharmaceutical Society of Japan
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