Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Physicochemical Characteristics and Antitumor Activities of a Highly Branched Fungal (1→3)-β-D-Glucan, OL-2, Isolated from Omphalia lapidescens
大野 尚仁三浦 敏秀斉藤 和夫西島 基弘宮崎 利夫宿前 利郎
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1992 年 40 巻 8 号 p. 2215-2218

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Physicochemical properties and antitumor activities of a fungal (1→3)-β-D-glucan, OL-2, isolated from Leiwan (Omphalia lapidescens) were examined. OL-2 showed sharp signals on carbon-13 nuclear magnetic resonance spectrum in dimethylsulfoxide-d6 as a solvent, and these signals were significantly reduced by the addition of distilled water to the concentration of 20%. This phenomenon is consistent with the general property of the gel forming (1→3)-β-D-glucan. Binding of OL-2 to Congo red induced a significant change of λmax to a longer wavelength, and the concentration to induce gel to sol transition was about 0.7 N; in contrast, the concentration was about 0.2 N in the cases of SPG and curdlan. These observations suggested that the gel structure would be significantly stabilized in the case of OL-2. OL-2 showed no or low antitumor activity against the solid form of Sarcoma 180 by intraperitoneal and intralesional administrations; however, it was effective on the ascites form of Sarcoma 180. Of interest, OL-2 also showed significant antitumor activity against the ascites form of MH-134 when administered with 5-fluorouracil. These results indicated that OL-2 showed characteristic features regarding its physicochemical properties and antitumor activity.

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