Cryobiology and Cryotechnology
Online ISSN : 2424-1555
Print ISSN : 1340-7902
Evaluation of the Improved Embryonic Membrane Permeability for Artemia Franciscana Hydrated Cysts
Miki MOROIKeisuke FUKUOToru YOSHIDA
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JOURNAL FREE ACCESS

2020 Volume 66 Issue 2 Pages 151-155

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Abstract

The cysts of Artemia franciscana (A. franciscana) exhibit intense tolerance to desiccation and freezing. However, when cysts are hydrated, these resistances to the extreme environments are drastically impaired. In this report, we investigated into improved membrane permeability of the decapsulated cysts by the treatments of synthetic cationic polymer and several amphipathic anionic surfactants. We found here that poly-allylamine, synthetic cationic polymer, was to be relatively effective on the embryonic membrane permeability of hydrated cysts. This reagent could prove useful when introducing alternative endogenous factors that assist their stress-resistant functions in future studies.

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© 2020 Japanese Society of Cryobiology and Cryotechnology
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