Abstract
A cell line of retinoblastoma cells, TOTL-1, was established which hada very weakly amplified N-myc gene. It grew only in clumps that were hardly ever dispersed and showed retarded proliferation. Its doubling time was 160 h. TOTL-1 cells extended short thick processes in monolayer cultures on poly-D-lysine coated substratum, which were markedly different from those of Y79 cells. Light and electron microscopy observation of immunostaining with anti-pp60srcsrc antibody revealed that the staining was accumulated in the polar regions of the cells.
