2005 年 70 巻 1 号 p. 59-64
Our aim is to develop and evaluate monitoring systems using aquatic organisms to assess the genotoxicity of water. We evaluated the genotoxicity of 5 model chemical mutagens by single cell gel electrophoresis (comet assay) using goldfish primary culture cells. The cells were collected at 3 h after treatment of each mutagen. Treatments with an alkylating agent (MNNG), a DNA oxidative agent (H2O2), a DNA crosslinking agent (MMC), an RNA polymerase inhibitor (Actinomycin D), and a topoisiomerase inhibitor (VP-16) induced DNA fragmentation. All of tested chemicals induced DNA damage fragmented by dose- and time-dependently except MMC. Since the comet assay detects genotoxicity as DNA fragmentation, this result shows that the comet assay using fish cells are suitable for the monitoring of environmental genotoxicity.
