Studies on the Cytogenetic and DNA Damage Induced by Theophylline in Different Tissues of Mice
2005 Volume 70 Issue 3 Pages 315-321
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2005 Volume 70 Issue 3 Pages 315-321
Theophylline (TH) is a methylxanthine widely used in clinical practice. The genotoxic effects of TH was investigated in mouse spleen, and spermatogonia cells. Also damage of DNA and molecular detection of apoptosis were applied. TH was administered orally as single dose of 25, 50, 75 and 120 mg kg−1 b.wt. and a multiple treatment with a daily dose of 25, 50 and 75 mg kg−1 b.wt. for 3 and 7 successive days. TH induced a significant increase in the percentage of chromosomal aberrations in somatic and germ cells which was dose and time dependent.
Also, TH induced DNA damage and apoptosis in a dose and time dependent manner in mouse liver cells. However, induced DNA damage and apoptosis was maximally detected at a concentration of 120 mg TH kg−1 b.wt. after oral treatment for 1 d.
