2006 年 71 巻 4 号 p. 331-335
The Random Amplified Polymorphic DNA (RAPD) technique was used to study DNA profiling of 5 multivoltine silkworm genotypes. The silkworm Bombyx mori. L, (Lepidoptera; Bombycidae) were analyzed using 30 random primers among which 18 polymorphic primers gave 73 amplified products and of which 38.3% were polymorphic. The dendrogram generated using an unweighted pair grouped method with arithmetic averages revealed the pattern of relatedness of 5 genotypes. Genetic similarity co-efficient and cluster analysis were performed by a hierarchical clustering technique. The genetic distances between the clusters and within the clusters estimated 6% variability between the 4 races and Nistari. The results of our study indicate that RAPDs are very efficient in the estimation of genetic diversity in populations that are closely related and acclimatized to local environmental conditions. The polymorphic data obtained from the study can be further utilized for MV genome mapping research and finally to assign function to sequences through biometrical tools. Modern breeding tools like molecular markers which show easily detectable differences among different races of a species offer a wide range of applications for silkworm breeding programs. India is being a country with diverse environmental conditions, the local races are rich reservoirs of many resistant genes, and molecular markers are inevitable tools to study inheritance of such complex genes.
