A Study of Nucleolus Organizing Regions by Ag-staining, in situ Hybridization and Chromomycin A3 Techniques in Tilapia rendalli (Cichlidae, Perciformes) of the River Iguaçu Basin, Paraná, Brazil

Sônia Maria Hiromi Kakagawa; Mizoguchi Ana Luiza de Brito Portela-Castro; Isabel Cristina Martins-Santos

doi:10.1508/cytologia.72.373

A Study of Nucleolus Organizing Regions by Ag-staining, in situ Hybridization and Chromomycin A₃ Techniques in Tilapia rendalli (Cichlidae, Perciformes) of the River Iguaçu Basin, Paraná, Brazil

Sônia Maria Hiromi Kakagawa, Mizoguchi Ana Luiza de Brito Portela-Castro, Isabel Cristina Martins-Santos

著者情報

キーワード: Ag-NOR banding, C banding, CMA₃, FISH, 18S rDNA, Tilapia rendalli

ジャーナルオープンアクセス

2007 年 72 巻 3 号 p. 373-378

DOI https://doi.org/10.1508/cytologia.72.373

詳細

抄録

Chromosomes of Tilapia rendalli from river Iguaçu, particularly its nucleolus organizing regions, was analyzed by Ag-staining NOR (Ag-NOR), Chromomicyn A₃ (CMA₃) and fluorescence in situ hybridization techniques (FISH). Karyotypic analyses showed diploid number 2n=44, with 10 submetacentric (SM), 8 subtelocentric (ST) and 26 acrocentric (A) chromosomes, and constitutive pericentromeric heterochromatin standard for most chromosomes. Whereas Ag-NOR and CMA₃ detected 2 chromosome pairs, FISH method revealed 3 chromosome pairs with fluorescent signs and, thus, additional 18S cistrons. Results show that additional rDNA sites was inactive. Whilst active pairs were heterochromatic and CMA₃ positive, the inactive one was heterochromatic and CMA₃ negative.

責任著者(Corresponding author)

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