2010 年 75 巻 1 号 p. 23-30
This work is the first description of a cytogenetic study of the Indian muntjac (Muntiacus muntjak) in Laos PDR. Blood samples were taken from 1 male and 1 female Indian muntjac kept in Laos Zoo, Laos PDR. Lymphocytes were cultured following standard methods. Cell harvesting was performed and followed by metaphase chromosome spreading on microscopic slides. Conventional staining, G-banding and high-resolution techniques were then applied using Giemsa's stain. The results showed that the number of diploid chromosomes (2n) of the Indian muntjac was 6 in female and 7 in male. The short arm of chromosome pair 3 referred to the X chromosome. Idiograms represent the banding pattern at the metaphase chromosome and prometaphase chromosome were 149 and 192 bands, respectively. The polymorphism of nucleolar organizer regions (NORs) was found at 2 sites on chromosome pair 1, and 1 site on chromosome pair 3 (heteromorphic, 3a3b) in the female, whereas they were found at 2 sites on chromosome pair 1 and 2 sites on chromosome pair 3 (homomorphic, 3a3a) in the male. These results will be useful for providing basic genetic information and future studies of the breeding, conservation and chromosome evolution in this animal.
